Increased translational potential via high quality and fresh on-demand human hepatocytes
In vitro experiments don’t have to produce results that are disconnected from human physiology. With PXB-cells®, you can model human cell biology in your in vitro experiments, enabling you to progress confidently to in vivo studies. Moreover, because PXB-cells® and PXB-mice have the same source of donor hepatocytes, you can directly compare between in vitro cell data and in vivo data collected from PXB-mice.
PXB-cells® are ~95% pure human hepatocytes freshly isolated from the PXB-mouse®. Their high human-specific enzyme and transporter activity can be maintained long-term, providing a superior in vitro model system of the human liver environment. Our stable supply of on-demand PXB-cells® enables you to perform reproducible experiments using the same source of donor hepatocytes.
PXB-cells® are ideally suited for studies which require the use of primary human hepatocytes, such as drug metabolism, enzyme induction/inhibition, safety assessment, transporter evaluation, anti-HBV therapeutics efficacy testing and other studies.
Can be infected with Hepatitis B Virus (HBV)
Produce high levels of human lipoproteins
Suitable for 3D cell culture or other culture systems
PXB-cells® have high and stable expression of human genes and proteins, enabling you to model how your drug performs in human hepatocytes. PXB-cells® demonstrate high and stable expression of important phase I and II metabolic enzymes and transporters:
PXB-cells® show stable, high levels of active human enzymes, meaning your drug candidates will be modelled in a functional human hepatocyte-based environment — enabling you to better predict its efficacy and toxicity in vivo.
CYP3A activity in PXB-cells |
|
Measured enzyme |
CYP3A |
Enzyme activity |
Midazolam 1’ – hydroxylase |
Matrix |
Midazolam (10μM) |
Processing time |
2 hours |
Metabolites |
1’ - hydroxymidazolam |
PhoenixBio delivers PXB-cells® in various plate and flask formats under temperature-controlled conditions (15-25°C). We also provide dHCGM media (-FBS) to maintain the optimal culture conditions of your fresh PXB-cells.
PXB-cells® supply process |
|
24-well plate |
1.0 x 107 cells per plate |
96-well plate |
6.5 x 106 cells per plate |
24-well plate |
1.6 x 107 cells per flask |
*Please note that you can re-plate PXB-cells® to your preferred formats from T75 flasks. We will provide a protocol for the plating procedures upon request.s
Our PXB-cells® have helped to drive hundreds of drug discovery projects over the last decade and enabled organizations to achieve accurate human liver data.
Contact us to find out how the PhoenixBio team could help your drug discovery and development projects today.