PXB-cells®
Increased translational potential via high quality and fresh on-demand human hepatocytes
In vitro experiments don’t have to produce results that are disconnected from human physiology. With PXB-cells®, you can model human cell biology in your in vitro experiments, enabling you to progress confidently to in vivo studies. Moreover, because PXB-cells® and PXB-mice have the same source of donor hepatocytes, you can directly compare between in vitro cell data and in vivo data collected from PXB-mice.
PXB-cells® are ~95% pure human hepatocytes freshly isolated from the PXB-mouse®. Their high human-specific enzyme and transporter activity can be maintained long-term, providing a superior in vitro model system of the human liver environment. Our stable supply of on-demand PXB-cells® enables you to perform reproducible experiments using the same source of donor hepatocytes.
Applications
PXB-cells® are ideally suited for studies which require the use of primary human hepatocytes, such as drug metabolism, enzyme induction/inhibition, safety assessment, transporter evaluation, anti-HBV therapeutics efficacy testing and other studies.
PXB-cells®: hepatocytes with human gene and enzyme expression
- Consistent supply from the same donor
- Long-term stability: up to 40 days or more
- Confirmed activities of enzymes and transporters
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Can be infected with Hepatitis B Virus (HBV)
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Produce high levels of human lipoproteins
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Suitable for 3D cell culture or other culture systems
Expression of human genes and enzymes in PXB-cells®
PXB-cells® have high and stable expression of human genes and proteins, enabling you to model how your drug performs in human hepatocytes. PXB-cells® demonstrate high and stable expression of important phase I and II metabolic enzymes and transporters:
Human-type enzyme activity in PXB-cells®
PXB-cells® show stable, high levels of active human enzymes, meaning your drug candidates will be modelled in a functional human hepatocyte-based environment — enabling you to better predict its efficacy and toxicity in vivo.
CYP3A activity in PXB-cells |
|
Measured enzyme |
CYP3A |
Enzyme activity |
Midazolam 1’ – hydroxylase |
Matrix |
Midazolam (10μM) |
Processing time |
2 hours |
Metabolites |
1’ - hydroxymidazolam |
PXB-cells® supply process
PhoenixBio delivers PXB-cells® in various plate and flask formats under temperature-controlled conditions (15-25°C). We also provide dHCGM media (-FBS) to maintain the optimal culture conditions of your fresh PXB-cells.
PXB-cells® supply process |
|
24-well plate |
1.0 x 107 cells per plate |
96-well plate |
6.5 x 106 cells per plate |
24-well plate |
1.6 x 107 cells per flask |
*Please note that you can re-plate PXB-cells® to your preferred formats from T75 flasks. We will provide a protocol for the plating procedures upon request.s
See how PXB-cells® have been used in other studies
Our PXB-cells® have helped to drive hundreds of drug discovery projects over the last decade and enabled organizations to achieve accurate human liver data.
Case study: Mitochondrial toxicity with PXB-cells®
- The mitochondrial function of cryopreserved human hepatocytes (CHH) and PXB-cells® were directly compared
- PXB-cells® outperformed CHH lots in terms of energy metabolism and mitochondrial function
Case study: PXB-cells® in metabolite identification
- PXB-cells® were used as a model in vitro system for the prediction of the in vivo metabolism of synthetic opiates: fentanyl and its analogue acetylfentanyl.
- The metabolite profiles in PXB-cells® were consistent with the previously reported in vivo metabolite profiles of drug users.
- Higher amounts of metabolites were formed in PXB-cells® when compared to another source of commercially available primary human hepatocytes (PHH). Some metabolites were detected only in the medium of PXB-cells®.
