PXB-cells®

Increased translational potential via high quality and fresh on-demand human hepatocytes

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In vitro experiments don’t have to produce results that are disconnected from human physiology. With PXB-cells®, you can model human cell biology in your in vitro experiments, enabling you to progress confidently to in vivo studies. Moreover, because PXB-cells® and PXB-mice have the same source of donor hepatocytes, you can directly compare between in vitro cell data and in vivo data collected from PXB-mice.

PXB-cells® are ~95% pure human hepatocytes freshly isolated from the PXB-mouse®. Their high human-specific enzyme and transporter activity can be maintained long-term, providing a superior in vitro model system of the human liver environment. Our stable supply of on-demand PXB-cells® enables you to perform reproducible experiments using the same source of donor hepatocytes.

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Applications

PXB-cells® are ideally suited for studies which require the use of primary human hepatocytes, such as drug metabolism, enzyme induction/inhibition, safety assessment, transporter evaluation, anti-HBV therapeutics efficacy testing and other studies.

Why use PXB-cells®?

PXB-cells®: hepatocytes with human gene and enzyme expression

  • Consistent supply from the same donor
  • Long-term stability: up to 40 days or more
  • Confirmed activities of enzymes and transporters
  • Can be infected with Hepatitis B Virus (HBV)

  • Produce high levels of human lipoproteins

  • Suitable for 3D cell culture or other culture systems 

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Expression of human genes and enzymes in PXB-cells®

PXB-cells® have high and stable expression of human genes and proteins, enabling you to model how your drug performs in human hepatocytes. PXB-cells® demonstrate high and stable expression of important phase I and II metabolic enzymes and transporters:

Human-type enzyme activity in PXB-cells®

PXB-cells® show stable, high levels of active human enzymes, meaning your drug candidates will be modelled in a functional human hepatocyte-based environment enabling you to better predict its efficacy and toxicity in vivo.

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CYP3A activity in PXB-cells

Measured enzyme

CYP3A

Enzyme activity

Midazolam 1’ – hydroxylase

Matrix

Midazolam (10μM)

Processing time

2 hours

Metabolites

1’ - hydroxymidazolam

PXB-cells® supply process

PhoenixBio delivers PXB-cells® in various plate and flask formats under temperature-controlled conditions (15-25°C). We also provide dHCGM media (-FBS) to maintain the optimal culture conditions of your fresh PXB-cells.

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PXB-cells® supply process

24-well plate

1.0 x 107 cells per plate

96-well plate

6.5 x 106 cells per plate

24-well plate

1.6 x 107 cells per flask

*Please note that you can re-plate PXB-cells® to your preferred formats from T75 flasks. We will provide a protocol for the plating procedures upon request.s

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See how PXB-cells® have been used in other studies

Our PXB-cells® have helped to drive hundreds of drug discovery projects over the last decade and enabled organizations to achieve accurate human liver data.

Case study: Mitochondrial toxicity with PXB-cells®

  • The mitochondrial function of cryopreserved human hepatocytes (CHH) and PXB-cells® were directly compared
  • PXB-cells® outperformed CHH lots in terms of energy metabolism and mitochondrial function
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Case study: PXB-cells® in metabolite identification

  • PXB-cells® were used as a model in vitro system for the prediction of the in vivo metabolism of synthetic opiates: fentanyl and its analogue acetylfentanyl.
  • The metabolite profiles in PXB-cells® were consistent with the previously reported in vivo metabolite profiles of drug users.
  • Higher amounts of metabolites were formed in PXB-cells® when compared to another source of commercially available primary human hepatocytes (PHH). Some metabolites were detected only in the medium of PXB-cells®.
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Get in Touch

Contact us to find out how the PhoenixBio team could help your drug discovery and development projects today.