The current experimental approach for studying alcohol-associated liver disease (ALD) has limitations due to insufficient expression and activity of alcohol-metabolizing enzymes in hepatocytes using standard cell culture systems and animal models. Even with a 3D in vitro organoid system, we experience non-physiological structure, inconsistent microenvironment, and uneven nutrient and oxygen supply. Therefore, we developed a Liver-on-a-Chip culture system from Emulate using and human liver cells (PXB-cells) from PhoenixBio, which express relevant levels of alcohol-metabolizing enzymes. There is an unmet need for a human-relevant in vitro 3D culture model to test new therapeutic approaches. In our initial study, we determined that ALDH2 is expressed in liver sinusoidal endothelial cells (LSEC) besides hepatocytes. Intriguingly, senescent LSECs showed reduced expression of ALDH2