NASH/NAFLD
Supporting your NASH/NAFLD drug development programs using a chimeric mouse model with a humanized liver.
The challenge
At PhoenixBio, we understand the complexities of building robust NASH/NAFLD drug development programs. With the disease’s many complex factors, it is challenging to discern underlying mechanisms and identify clinically significant biomarkers. Currently available animal models tend to lack key disease characteristics such as hepatocyte ballooning/fibrosis, and have lengthy timescales in presenting fibrosis after high-fat diets.
PXB-mice: a new translational model for NASH
Our unique chimeric PXB-mouse® with a humanized liver is a next-generation research tool that exhibits human NASH-like disease related histopathological changes including hepatocyte ballooning, mallory-denk bodies, perisinusoidal fibrogenesis, and inflammation — offering you a comprehensive validation method for pre-clinical NASH/NAFLD research studies.
The PXB-mouse® allows you to better understand the human liver condition with NASH/NAFLD, identify clinically significant biomarkers to better predict the disease and bring therapeutic agents to market.
Induce the development of NASH in as little as 12 weeks
Induce human-like NASH symptoms in PXB from 12 weeks with the choline-deficient, high-fat diet (CBAHFD), and 20-30 weeks with the Gubra-Amylin Nash (GAN) diet, closely resembling the slow onset of fibrosis caused by the ‘Western diet’ in humans.
Left Panel: Control (Regular diet);
Right Panel: Choline deficient, L amino acid defined, high fat diet. H&E images at 12 weeks of diet administration. Arrows indicate hepatocyte ballooning and Mallory-Denk Bodies.
Image from: Papazyan et al., J Lipid Res. 2018 Mar 20
Test your therapeutic in a model that has a humanized lipoprotein profile
PXB-mice have a chimeric humanized liver environment containing up to 97% human hepatocytes. Their human-like lipoprotein profile makes them an ideal model to test human outcomes of NASH/NAFLD drug development programs.
Recapitulate the NASH phenotype observed in humans in our chimeric mouse model with a humanized liver. Our PXB-mice fed on a high-fat diet show human-like hepatocyte ballooning and other characteristics not typically observed in rodents.
Target fibrosis in a humanized liver chimeric mouse model
Recapitulate the NASH phenotype observed in humans in our chimeric mouse model with a humanized liver. Our PXB-mice are fed a high-fat diet show human-like hepatocyte ballooning and other characteristics not typically observed in rodents.
Control (Regular diet);
NASH (Choline deficient, L amino acid defined, high fat diet). Sirius Red staining images at 8-weeks and 12-weeks post diet administration. No collagen fibrils were observed in the Control group, while perisinusoidal fibrosis was observed in the NASH groups.